订购/支持:theta_gene@163.com
公司网址:https://www.theta-gene.com
全球最大CRISPR工具平台(蛋白/mRNA)
The World's Largest Precision Gene/Base/Prime Editing Arsenal
-80℃储存,低温运输。
产品编号 | 产品名称 | 包装 |
N1013 | Cas12i1 mRNA (1mg/ml) | 100/1000μg |
来源于毛螺菌科细菌Lachnospiraceae bacterium ND2006的V-I型CRISPR-Cas12核酸酶。
优先切割双链DNA的非靶链(NTD strand),效率显著高于靶链(TD strand),切割位点位于PAM下游13-15 nt(非靶链)和24 nt(靶链),形成交错双链断裂。
通过"拉链基序"(Helical-I结构域160-177aa)和两对"磷酸基团锁"(R12/K483、R535/K923)促进DNA解旋,PI结构域的G235/A236残基决定PAM识别(如5'-TTN-3')。
分子量约1093氨基酸,比Cas9(1368aa)和Cas12a更紧凑,适配单AAV载体递送。
无需tracrRNA,可自主加工pre-crRNA生成成熟crRNA,sgRNA长度仅43-45 nt。
CDS (3282nt):
atgtctaacaaagaaaaaaatgcaagcgaaactcgcaaagcctacacaacaaaaatgattccaagaagccatgatcgcatgaaattgcttgggaatttcatggattatttgatggatggaacgccaatatttttcgaactttggaatcagttt
ggcggcgggattgaccgcgatatcatttctggcactgcaaataaagacaagatatcagatgatttacttttggcggtcaattggttcaaggtaatgccaattaattctaagcctcaaggtgtatcgccatcaaatcttgccaacctctttcaaca
atactctggatcagaaccagacattcaagctcaagagtattttgcttcaaattttgacaccgaaaagcatcaatggaaggacatgcgtgttgaatacgaacgactattagctgaattgcagctatcgagaagtgatatgcatcatgacttgaa
gctcatgtacaaagaaaaatgcattggcctaagtctttctacggctcactacatcacttctgtgatgtttgggacaggagctaaaaacaatcgccaaaccaagcatcaattctatagcaaggttatccaactacttgaggaatcaactcaaat
caattctgttgaacagttggcatctattattttgaaagcaggagattgcgatagttatcgaaagcttcgtattcgatgttctcgtaagggagcaacacccagcattcttaagatcgttcaagactatgaactgggaaccaatcacgatgatgaa
gtgaatgtgccaagtttgattgcaaatttgaaagaaaaattgggcagatttgaatatgaatgcgaatggaagtgcatggaaaaaatcaaagcatttttagctagcaaagttgggccttattacctaggctcttacagtgcgatgcttgaaaatg
cattgtcgcccatcaagggaatgactacaaaaaattgcaaatttgtgttaaagcaaattgatgccaaaaacgacatcaagtatgaaaatgagccatttggcaaaattgttgaagggttttttgactctccatattttgaaagcgacaccaatgt
gaaatgggttttgcacccacatcatattggagaaagcaatatcaaaacactctgggaagacttgaatgcaattcattctaagtacgaagaagatattgcttctttgagcgaagacaaaaaagagaaacgcattaaggtttatcaaggagatg
tttgccaaacaatcaatacgtattgtgaagaagtaggaaaggaagctaagactcctttagttcagcttttgcgttatctttactctagaaaagatgatattgctgttgataagataattgatggcattaccttccttagcaagaaacacaaggttga
aaaacaaaaaatcaatcctgtaattcaaaaatatcccagtttcaactttgggaataattctaagttgttgggaaagattatcagccccaaagacaagttaaagcataatctcaaatgcaacaggaatcaggttgataattacatttggattgaga
ttaaag………….
可选项:
