-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1075 | Sth1Cas9 mRNA (1mg/ml) | 100/1000μg |
产品简介：
II类Cas9
肽链长度约1130 aa
双链DNA切割活性；PAM为NNGGAA
在多种链霉菌（Streptomyces）中功能良好，尤其适用于SpCas9失效菌株
HDR介导的定点敲入和缺失；用于天然产物生物合成基因簇的精确修饰、发现与多样化
CDS (3516nt):
atgtcggacctggtgctgggcctggacatcggcatcggctccgtcggcgtcggcatcctgaacaaggtgaccggcgagatcatccacaagaactcccgcatcttcccggccgcacaggccgagaacaacctggtccgccgcacca
accgccagggccgccgcctggcccgccgcaagaagcaccgccgcgtccgcctgaaccgcctgttcgaggagtccggcctgatcaccgacttcaccaagatcagcatcaacctgaacccgtaccagctgcgcgtcaagggcctga
ccgacgagctgtccaacgaggagctgttcatcgccctgaagaacatggtcaagcaccgcggcatctcctacctggacgacgcctccgacgacggcaactcctccgtcggcgactacgcccagatcgtcaaggagaactccaagca
gctggaaaccaagacccccggccagatccagctggagcgctaccagacctacggccagctgcgcggcgacttcaccgtcgagaaggacggcaagaagcaccgcctgatcaacgtgttcccgacctccgcctaccgctccgagg
ccctgcgcatcctccagacccagcaggagttcaacccgcagatcaccgacgagttcatcaaccgctacctggagatcctgaccggcaagcgcaagtactaccacggccccggcaacgagaagtcccgcaccgactacggccgcta
ccgcacctccggcgagacgctggacaacatcttcggcatcctgatcggcaagtgcaccttctacccggacgagttccgcgccgccaaggcctcctacaccgcccaggagttcaacctgctgaacgacctgaacaacctgaccgtcc
cgaccgagacgaagaagctgtccaaggagcagaagaaccagatcatcaactacgtcaagaacgagaaggcgatgggcccggccaagctgt………….
参考文献
Yeo, W. L. et al. Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes. Biotechnol. Bioeng. 116, 2330–2338 (2019).
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