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全球最大CRISPR工具平台(蛋白/mRNA

The World's Largest Precision Gene/Base/Prime Editing Arsenal

evoCas9 mRNA

evoCas9 mRNA

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货号:
  

-80℃储存,低温运输。

 

包装内容:

产品编号

产品名称

包装

N1081

evoCas9 mRNA (1mg/ml)

100/1000μg

 

产品简介:

名称/别名:evoCas9

CRISPR类型:Type II-ASpCas9衍生)

肽链长度:1,368 aa(与WT SpCas9一致)

活性:dsDNA双链切割,最佳活性需Mg²⁺;在59个靶位点测试中仅检出4个脱靶位点,特异性最高

特性:

高保真突变组合体,脱靶率<0.7%WT0.7%

保持与WT相当的on-target活性

产生WT类似的+1插入频率,用于保证编码区移码

应用:高特异性基因敲除、临床级基因编辑、+1插入介导的移码修复

CDS (4272nt):

atggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccgacaagaagtacagcatcggcctgg

acatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagcgctgctgttcgacagcggcgaaa

cagccgaggccacccggcttaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcct

ggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgat

ctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgc

cagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctgacccccaa

cttcaagagcaacttcgacctggccgaggatgccaaact………….

 

参考文献

Schmid-Burgk, J. L. et al. Highly parallel profiling of Cas9 variant specificity. Mol. Cell 78, 4 (2020).

 

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