Hsp1Cas9 mRNA

-80℃储存，低温运输。

包装内容：

产品简介：

名称/别名：Type II-C

肽链长度：1,057 aa

活性：dsDNA endonuclease；最佳活性需Mg²⁺

特性：识别N4RAA PAM；在人类细胞中活性高于Hsp2/Ccu；对PAM-远端错配容忍度高

应用：哺乳动物基因敲除、PAM扩展工具

CDS (3231nt):

atggccccgaagaaaaagcgcaaggtcgaagcgtccagcgacaaatccaagatcctgggattcgacatcggcatcaccagcatcggctgggcctacgtggaaggcgaagagctgatcgactgcggcgtccggatctttaccaagg

ccgagaatcccaagaccaaagaatccctggccctgcctagacgggaagctagaggcgtgcggcggagactggcccgaagaaaaggcagactgaatgccatcaagcaactgatctgcaaggaattcggcctgaactaccaagact

acatcgctcaggacggcgagctgcccaaagcctacgccgggaagctgattaacccttatgagctgagatctaaggcccttagagagaagctggaaaaggaagagctggcccgagtgattctgcacatcgccaagcaccggggcta

cggaaacaagcatgccaaggaaaccgacgatgccgagagcggcaagatcaagaaagcaattagcaacaatgagaagatcctgaatgagaagggatatcagagcgccggagagtacctgtaccaggaatttttccagaaagaaag

actgcaggaggaaaagaagggcaacaacacgaccaaagaattcaaaaatgtgaggaacaaagctggcgactacgagcactgtatcgctcagacccagatgcagaacgagttgaggcttattttcgagaagcagcgagagtttggcc

tgaacttcagcgaggaattcaagaagaaagtgctggagacggccttttttcagagacctctgaaagacttcagcgataagatcggcgattgcaccttcatcgagggcgccaagcgggcccctaaggacagcctgagcgccatcgagtt

cgtggccctgggaagaatcatcaacaccctgaaaaatctgcagaacacaagcggcgaggtgtacagcagagataagatcaacgagatcctgcaaatcgtgctggataagggcgagatcagctacaagaaactgagagaaatcctg

gacatcgaga………….

参考文献

Gao, S. et al. Genome editing with natural and engineered CjCas9 orthologs. Mol. Ther. 31, 1177–1187 (2023).

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