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全球最大CRISPR工具平台(蛋白/mRNA

The World's Largest Precision Gene/Base/Prime Editing Arsenal

SlugCas9 mRNA

SlugCas9 mRNA

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货号:
  

-80℃储存,低温运输。

 

包装内容:

产品编号

产品名称

包装

N1089

SlugCas9 mRNA (1mg/ml)

100/1000μg

 

产品简介:

名称/别名: SlugCas9(路邓葡萄球菌来源的Cas9核酸酶)

CRISPR类型: II型(II-A亚型)

肽链长度: 1,054个氨基酸(aa

活性: 核酸内切酶活性(基因组编辑、碱基编辑)

PAM识别:NNGG(图2B,C

高活性:在HEK293T等哺乳动物细胞中编辑效率与SaCas9相当(图3C,D

高保真变体:SlugCas9-HFR247A/N415A/T421A/R656A突变)显著降低脱靶(图4A,C

多功能工程化:可构建碱基编辑器(SlugBE4max诱导CT转换,SlugABEmax诱导AG转换);工程化变体SlugCas9-KHQ782K/R1014H)扩展PAMNNRG

应用:

CDS (3417nt):

atgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactaccggtgccaccatggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccaaccaaaaattcatactgggactgga

catcggaatcaccagcgtgggctacggcctgatcgactacgagacaaagaatatcatcgatgccggcgttagactgttccccgaggccaacgtggaaaacaacgagggaagaaggtccaaacgtggaagcagaagactgaagcg

acgccgcattcacagacttgaacgggtgaagaagctgctcgaggattataatctgctggatcagtcccagattcctcagtctacaaacccctacgccatccgcgtgaagggcctgtctgaagccctgagcaaggacgaactcgtgattg

ccctgctccatatcgccaagagaagaggcatccacaagatcgacgtgatcgacagcaacgacgacgtggggaacgagctcagcaccaaggaacagctgaataagaacagcaagctgctgaaagacaaatttgtgtgccagatcca

gctggaaagaatgaatgagggccaggtgcggggagagaaaaaccggttcaagaccgctgatatcatcaaggaaatcatccagctgctgaatgtgcagaagaacttccaccagctggacgagaacttcatcaacaagtacatcgaact

ggttgagatgagacggg………….

 

参考文献

Hu, Z. et al. Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity. Nucleic Acids Res. 49, 4008-4019 (2021).

 

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