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全球最大CRISPR工具平台(蛋白/mRNA)
The World's Largest Precision Gene/Base/Prime Editing Arsenal
-80℃储存,低温运输。
产品编号 | 产品名称 | 包装 |
N1089 | SlugCas9 mRNA (1mg/ml) | 100/1000μg |
名称/别名: SlugCas9(路邓葡萄球菌来源的Cas9核酸酶)
CRISPR类型: II型(II-A亚型)
肽链长度: 1,054个氨基酸(aa)
活性: 核酸内切酶活性(基因组编辑、碱基编辑)
PAM识别:NNGG(图2B,C)
高活性:在HEK293T等哺乳动物细胞中编辑效率与SaCas9相当(图3C,D)
高保真变体:SlugCas9-HF(R247A/N415A/T421A/R656A突变)显著降低脱靶(图4A,C)
多功能工程化:可构建碱基编辑器(SlugBE4max诱导C→T转换,SlugABEmax诱导A→G转换);工程化变体SlugCas9-KH(Q782K/R1014H)扩展PAM至NNRG
应用:
CDS (3417nt):
atgggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactaccggtgccaccatggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccaaccaaaaattcatactgggactgga
catcggaatcaccagcgtgggctacggcctgatcgactacgagacaaagaatatcatcgatgccggcgttagactgttccccgaggccaacgtggaaaacaacgagggaagaaggtccaaacgtggaagcagaagactgaagcg
acgccgcattcacagacttgaacgggtgaagaagctgctcgaggattataatctgctggatcagtcccagattcctcagtctacaaacccctacgccatccgcgtgaagggcctgtctgaagccctgagcaaggacgaactcgtgattg
ccctgctccatatcgccaagagaagaggcatccacaagatcgacgtgatcgacagcaacgacgacgtggggaacgagctcagcaccaaggaacagctgaataagaacagcaagctgctgaaagacaaatttgtgtgccagatcca
gctggaaagaatgaatgagggccaggtgcggggagagaaaaaccggttcaagaccgctgatatcatcaaggaaatcatccagctgctgaatgtgcagaagaacttccaccagctggacgagaacttcatcaacaagtacatcgaact
ggttgagatgagacggg………….
Hu, Z. et al. Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity. Nucleic Acids Res. 49, 4008-4019 (2021).
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