-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1124 | AspCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：AspCas12a（Acidaminococcus sp. Cas12a）
CRISPR类型：Type V
活性：高效基因组编辑
特性：识别TTTV PAM；依赖单crRNA引导；切割产生5'粘性末端
应用：人类细胞系（HEK293T、Jurkat、K562）、原代细胞（NK细胞、CD34+ HSPC）基因组编辑
CDS (4110nt):
atgacacagttcgagggctttaccaacctgtatcaggtgagcaagacactgcggtttgagctgatcccacagggcaagaccctgaagcacatccaggagcagggcttcatcgaggaggacaaggcccgcaatgatcactacaagga
gctgaagcccatcatcgatcggatctacaagacctatgccgaccagtgcctgcagctggtgcagctggattgggagaacctgagcgccgccatcgactcctatagaaaggagaaaaccgaggagacaaggaacgccctgatcgag
gagcaggccacatatcgcaatgccatccacgactacttcatcggccggacagacaacctgaccgatgccatcaataagagacacgccgagatctacaagggcctgttcaaggccgagctgtttaatggcaaggtgctgaagcagctg
ggcaccgtgaccacaaccgagcacgagaacgccctgctgcggagcttcgacaagtttacaacctacttctccggcttttatgagaacaggaagaacgtgttcagcgccgaggatatcagcacagccatcccacaccgcatcgtgcag
gacaacttccccaagtttaaggagaattgtcacatcttcacacgcctgatcaccgccgtgcccagcctgcgggagcactttgagaacgtgaagaaggccatcggcatcttcgtgagcacctccatcgaggaggtgttttccttcccttttta
taaccagctgctgacacagacccagatcgacctgtataaccagctgctgggaggaatctctcgggaggcaggcaccgagaagatcaagggcctgaacgaggtgctgaatctggccatccagaagaatgatgagacagcccacatc
atcgcctccctgccac ………….
参考文献
K. Luk et al. Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells. GEN Biotechnol. 2022, 1, 271–284.
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