-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1132 | dAsCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：催化失活型AsCpf1（dAsCpf1）
CRISPR类型：Type V-A（失活变体）
保留PAM依赖的靶标DNA结合能力
无核酸酶切割活性
通过关键催化残基突变（如D917A/E1006A）失活
保留PAM识别功能（TTTV）
用作阴性对照评估背景活性
CDS (4059nt):
atgacacagttcgagggctttaccaacctgtatcaggtgagcaagacactgcggtttgagctgatcccacagggcaagaccctgaagcacatccaggagcagggcttcatcgaggaggacaaggcccgcaatgatcactacaagga
gctgaagcccatcatcgatcggatctacaagacctatgccgaccagtgcctgcagctggtgcagctggattgggagaacctgagcgccgccatcgactcctatagaaaggagaaaaccgaggagacaaggaacgccctgatcgag
gagcaggccacatatcgcaatgccatccacgactacttcatcggccggacagacaacctgaccgatgccatcaataagagacacgccgagatctacaagggcctgttcaaggccgagctgtttaatggcaaggtgctgaagcagctg
ggcaccgtgaccacaaccgagcacgagaacgccctgctgcggagcttcgacaagtttacaacctacttctccggcttttatgagaacaggaagaacgtgttcagcgccgaggatatcagcacagccatcccacaccgcatcgtgcag
gacaacttccccaagtttaaggagaattgtcacatcttcacacgcctgatcaccgccgtgcccagcctgcgggagcactttgagaacgtgaagaaggccatcggcatcttcgtgagcacctccatcgaggaggtgttttccttcccttttta
taaccagctgctgacacagacccagatcgacctgtataaccagctgctgggaggaatctctcgggaggcaggcaccgagaagatcaagggcctgaacgaggtgctgaatctgg………….
参考文献
E. Tóth et al. Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 46, 10277–10291 (2018).
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