-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1133 | FnoCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：FnoCas12a（Francisella novicida Cas12a）
CRISPR类型：Type V-A
在哺乳动物细胞和斑马鱼胚胎中诱导靶向基因组编辑
依赖crRNA加工活性提升编辑效率
识别TTN PAM序列（较其他Cas12a更宽松）
通过工程化改造（如优化核定位信号、crRNA设计）可显著提升活性
依赖RNase活性处理前体crRNA以增强功能
CDS (4059nt):
atgagcatctaccaggagttcgtcaacaagtattcactgagtaagacactgcggttcgagctgatcccacagggcaagacactggagaacatcaaggcccgaggcctgattctggacgatgagaagcgggcaaaagactataagaa
agccaagcagatcattgataaataccaccagttctttatcgaggaaattctgagctccgtgtgcatcagtgaggatctgctgcagaattactcagacgtgtacttcaagctgaagaagagcgacgatgacaacctgcagaaggacttcaa
gtccgccaaggacaccatcaagaaacagattagcgagtacatcaaggactccgaaaagtttaaaaatctgttcaaccagaatctgatcgatgctaagaaaggccaggagtccgacctgatcctgtggctgaaacagtctaaggacaat
gggattgaactgttcaaggctaactccgatatcactgatattgacgaggcactggaaatcatcaagagcttcaagggatggaccacatactttaaaggcttccacgagaaccgcaagaacgtgtactccagcaacgacattcctacctcc
atcatctaccgaatcgtcgatgacaatctgccaaagttcctggagaacaaggccaaatatgaatctctgaaggacaaagctcccgaggcaattaattacgaacagatcaagaaagatctggctgaggaactgacattcgatatcgactat
aagactagcgaggtgaaccagagggtcttttccctggacgaggtgtttgaaatcgccaatttcaacaattacctgaaccagtccggcattactaaattcaataccatcattggcgggaagtttgtgaacggggagaataccaagc……
…….
参考文献
Liu, P. et al. Enhanced Cas12a editing in mammalian cells and zebrafish. Nucleic Acids Res. 47, 4169–4180 (2019).
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