-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1139 | BsCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：BsCas12a（Bacillus sp. Cas12a）
CRISPR类型：Type V-A
肽链长度：约1200-1300个氨基酸（aa）
识别5'-TTN PAM并介导DNA切割
在crRNA支架4n96优化下编辑效率显著提升
低脱靶风险（经全基因组测序验证）
与人工crRNA支架兼容
CDS (4566nt):
atgcccaagaagaagaggaaggttcccgggatgtactaccagaacctgaccaagaagtaccccgtgagcaagaccatccgcaacgagctgatccccatcggcaagaccctggagaacatccgcaagaacaacatcctggagagc
gacgtgaagcgcaagcaggactacgagcacgtgaagggcatcatggacgagtaccacaagcagctgatcaacgaggccctggacaactacatgctgcccagcctgaaccaggccgccgagatctacctgaagaagcacgtgga
cgtggaggaccgcgaggagttcaagaagacccaggacctgctgcgccgcgaggtgaccggccgcctgaaggagcacgagaactacaccaagatcggcaagaaggacatcctggacctgctggagaagctgcccagcatcagc
gaggaggactacaacgccctggagagcttccgcaacttctacacctacttcaccagctacaacaaggtgcgcgagaacctgtacagcgacgaggagaagagcagcaccgtggcctaccgcctgatcaacgagaacctgcccaagt
tcctggacaacatcaagagctacgccttcgtgaaggccgccggcgtgctggccgactgcatcgaggaggaggagcaggacgccctgttcatggtggagaccttcaacatgaccctgacccaggagggcatcgacatgtacaactac
cagatcggcaaggtgaacagcgccatcaacctgtacaaccagaagaaccacaaggtggaggagttcaagaagatccccaagatgaaggtgctgtacaagcagatcctgagcgaccgcgaggaggtgttcatcggcgagttcaag
gacgacgagaccctgctgagcagcatcggcgcctacggcaacgtgctgatgac………….
参考文献
Teng, F. et al. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Nat. Commun. 10, 1234 (2019).
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