-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1140 | HkCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：HkCas12a（Herpetosiphon sp. Cas12a）
CRISPR类型：Type V-A
肽链长度：约1200-1300个氨基酸（aa）
识别非经典PAM（5'-YTN/TYYN）
显著扩展基因组靶向范围
因L642残基变异实现宽PAM识别
基因组覆盖能力优于AsCas12a
CDS (4878nt):
atgcccaagaagaagaggaaggttcccgggatgttcgagaagctgagcaacatcgtgagcatcagcaagaccatccgcttcaagctgatccccgtgggcaagaccctggagaacatcgagaagctgggcaagctggagaaggac
ttcgagcgcagcgacttctaccccatcctgaagaacatcagcgacgactactaccgccagtacatcaaggagaagctgagcgacctgaacctggactggcagaagctgtacgacgcccacgagctgctggacagcagcaagaagg
agagccagaagaacctggagatgatccaggcccagtaccgcaaggtgctgttcaacatcctgagcggcgagctggacaagagcggcgagaagaacagcaaggacctgatcaagaacaacaaggccctgtacggcaagctgttc
aagaagcagttcatcctggaggtgctgcccgacttcgtgaacaacaacgacagctacagcgaggaggacctggagggcctgaacctgtacagcaagttcaccacccgcctgaagaacttctgggagacccgcaagaacgtgttcac
cgacaaggacatcgtgaccgccatccccttccgcgccgtgaacgagaacttcggcttctactacgacaacatcaagatcttcaacaagaacatcgagtacctggagaacaagatccccaacctggagaacgagctgaaggaggccg
acatcctggacgacaaccgcagcgtgaaggactacttcacccccaacggcttcaactacgtgatcacccaggacggcatcgacgtgtaccaggccatccgcggcggcttcaccaaggagaacggcgagaaggtgcagggcatca
acgagatcctgaacctgacccagcagcagctgcgccgcaagcccgagaccaagaac………….
参考文献
Teng, F. et al. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Nat. Commun. 10, 1234 (2019).
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