-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1141 | LpCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：LpCas12a（Lachnospiraceae bacterium Cas12a）
CRISPR类型：Type V-A
肽链长度：约1200-1300个氨基酸（aa）
依赖5'-TTTN PAM介导编辑
与人工crRNA支架（scaffold 6）协同提升效率
核定位明确（免疫荧光验证）
低脱靶率（Cas-OFFinder预测验证）
CDS (4866nt):
atgcccaagaagaagaggaaggttcccgggatgatcatgaacaacgtgaccggcgacttcagcgagttcgtggccatcagcaaggtgcagaagaccctgcgcaacgagctgcgccccacccccctgaccatgaagcacatcaag
cagaagggcatcatcaccgaggacgagtacaagacccagcagagcctggagctgaagcgcatcgccgacggctactaccgcgactacatcacccacaagctgaacgacaccaacaacctggacttccgcaacctgttcgaggcc
atcgaggagaagtacaagaagaacgacaaggacaaccgcgacaagctggacctggtggagaagagcaagcgcggcgagatcgccaagctgctgagcgccgacgacaacttcaagagcatgttcgaggccaagctgatcaccc
agctgctgcccgtgtacgtggagcagaactacatcggcgaggacaaggagaaggccctggagaccatcgccctgttcaagggcttcaccacctacttcaccgactacttcaacatccgcaagaacatgttcaaggagaacggcggc
gccagcagcatctgctaccgcatcgtgaacgtgaacgccagcatcttctacgacaacctgaagaccttcatgtgcatcaaggagaaggccgagaccgagatcgccctgatcgaggaggagctgaccgagctgctggacagctggc
gcctggagcacatcttcagcgaggactactacaacgagctgctggcccagaagggcatcgactactacaaccagatctgcggcgacgtgaacaagcacatgaacctgtactgccagcagaacaagctgaaggccaacgtgttcaag
atgaccaagctgcagaagcagatcatgggcatcagcgagaaggccttcgagatccc………….
参考文献
Teng, F. et al. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Nat. Commun. 10, 1234 (2019).
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