-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1142 | PrCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：PrCas12a（Prevotella sp. Cas12a）
CRISPR类型：Type V-A
肽链长度：约1200-1300个氨基酸（aa）
识别5'-TTN PAM
crRNA支架4n96可大幅提升编辑效率
与人工crRNA支架（scaffold 7）兼容
编辑效率经GFP报告系统验证
CDS (4587nt):
atgcccaagaagaagaggaaggttcccgggatgatcatcggccgcgacttcaacatgtactaccagaacctgaccaagatgtaccccatcagcaagaccctgcgcaacgagctgatccccgtgggcaagaccctggagaacatcc
gcaagaacggcatcctggaggccgacatccagcgcaaggccgactacgagcacgtgaagaagctgatggacaactaccacaagcagctgatcaacgaggccctgcagggcgtgcacctgagcgacctgagcgacgcctacga
cctgtacttcaacctgagcaaggagaagaacagcgtggacgccttcagcaagtgccaggacaagctgcgcaaggagatcgtgagcctgctgaagaaccacgagaacttccccaagatcggcaacaaggagatcatcaagctgctg
cagagcctgtacgacaacgacaccgactacaaggccctggacagcttcagcaacttctacacctacttcagcagctacaacgaggtgcgcaagaacctgtacagcgacgaggagaagagcagcaccgtggcctaccgcctgatca
acgagaacctgcccaagttcctggacaacatcaaggcctacgccatcgccaagaaggccggcgtgcgcgccgagggcctgagcgaggaggaccaggactgcctgttcatcatcgagaccttcgagcgcaccctgacccaggac
ggcatcgacaactacaacgccgccatcggcaagctgaacaccgccatcaacctgttcaaccagcagaacaagaagcaggagggcttccgcaaggtgccccagatgaagtgcctgtacaagcagatcctgagcgaccgcgaggag
gccttcatcgacgagttcagcgacgacgaggacctgatca………….
参考文献
Teng, F. et al. Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds. Nat. Commun. 10, 1234 (2019).
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