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全球最大CRISPR工具平台(蛋白/mRNA)
The World's Largest Precision Gene/Base/Prime Editing Arsenal
-80℃储存,低温运输。
产品编号 | 产品名称 | 包装 |
N1152 | OsCas12f1 mRNA (1mg/ml) | 100/1000μg |
名称/别名:OsCas12f1(Oscillibacter sp. Cas12f1)
CRISPR类型:Type V-F
原始编辑效率中等(1-20%),工程化后(enOsCas12f1)显著提升(9.4倍于野生型)
高效诱导大片段缺失(如DMD外显子51缺失)
识别5'-YTTH(Y=C/T, H≠G)PAM
蛋白尺寸超紧凑(433 aa),可单AAV递送
工程化变体(enOsCas12f1)通过精氨酸突变和sgRNA优化增强活性
CDS (1443nt):
atggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatgcctaagaagaagagaaaggtgatgggaaagggcgtgctggccaaggtgatgaaatacgagctgagatacctgga
tggttgtggcgacttcagcaatatgcaggagcaggtgtgggccctgcagcggcagacacgggaaatcctgaacagatccatccaaatcgccttccaatgggactgcgccaacagcgagcaccacagaaagaccggcgagtacctg
gacctgaaaacggaaaccggctacaagagactggatggccacatctacaactgcctgaagggccagtacgaggacatggccacatctaacctgaacgccaccatccagaaggcttggaagaagtataactccagcaagaaggaaa
tcctgaggggcagcatgagcatccccagctacaagatgaaccagcctctgacactggacaagaataccgtgaaactgtctgagggcgagcggaacccaatcgtgaccctgacactgtttagcgacaagttcaagcgggcccagggc
gtgtccaacgtgaagtttagcatgcctctgcacgacggcacccagagagccatcttcgccaacctgatgaacggcacctaccagctgggagagtgccagctggtgtacaaacggcctaagtggttcctgttcgtgacatacaagttccc
ccccgtggaacatcctctcgatcctgacaagattctgggcgtcgacatgggcgaggcctgcgcgctttatgcctctacattcggcgagcacggctacctgaagatcgatggaggcgagattacaaagtacgccaagaagatggaagct
agaatccggagcatgcagaagcaggctgctcactgtggcgaaggcagaatcgggcacggcaccaaaacaagagtgtctgtggtgtaccaggccaaggacaaggtggccagattcagagataccatcaac………….
Kong, X. et al. Engineered CRISPR-OsCas12f1 and RhCas12f1 with activities and expanded target range for genome editing. Nat. Commun. 14, 2836 (2023).
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