-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1153 | RhCas12f1 mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：RhCas12f1（Ruminiclostridium herbifermentans Cas12f1）
CRISPR类型：Type V-F
原始编辑效率中等（1-20%），工程化后（enRhCas12f1）提升1.7倍
高效靶向5'-CCD（D≠C）PAM位点
识别5'-CCD PAM，与OsCas12f1形成PAM互补
蛋白尺寸超紧凑（415 aa），单AAV递送兼容
工程化变体（enRhCas12f1）通过L270R突变和sgRNA优化增强活性
CDS (1389nt):
atggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatgcctaagaagaagagaaaggtgatgattacagtgcggaagctgaagattctgatcgacggcgagagcaggaacga
gagctacaagttcatccgggacagcatgtacgcccagtacctggccctgaacaaggccatgagctacctgggtaccgcctacctgagcagagataaggagatcttcaaggaggccatcaagtctctcaacaactctaatcctatcttcg
acaacattaacttcggcaagggaatcgataccaagagcagcgtgaaccagacagtgaagaaacacatccaggccgacattaagaatggcctggccaagggcgaaagatccatccggaactacaagcgtgactaccccctgatgac
cagaggcagagatctgaagtttttctactgcgacaccaatagcaccaaggtgaaggtgaaatgggtgaacggcatcatctttgacgtgatgctgggaaaggaatacaacaagaatgatctagagctgagatcttttctgaacagagtcat
caacaaggaatataagatctcccaaagcagcatctgcttcgacaagcacaacagactgatcctgaatctgagcgtgaacatcaccgacaacatccccaacgaggtggtgaagggcagaattgtgggcgtcgacctgggcatgaaaat
cccagcttatgtgacactgaacgacagcgagtacatcggaaaacctatcggcgacatcaacgacttcctgaaggtgcggaagcagttcaaagaaagaaaggagcggctgcagaagcagctggccatcaacaagggcggcagagg
catcaccaacaagatgcagctgatggacgccttcaccaacaaggaaaagaacttcgctaatacctacaaccacggcgtttctaaggcaatcatcaactttgctaagaagtacaaggccgagcagatcaacgtggagttcctggctctgg
ccggcagcgagaaggaaatcctgagctccacaatccgctactggtcctactatcaactgcaacagatgatcgagtacaa………….
参考文献
Kong, X. et al. Engineered CRISPR-OsCas12f1 and RhCas12f1 with activities and expanded target range for genome editing. Nat. Commun. 14, 2836 (2023).
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