-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1156 | dFnCas12a mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：dFnCas12a（失活型Francisella novicida Cas12a）
CRISPR类型：Type V-A
肽链长度：未提供（基于Cas12a家族估算约1200-1300 aa）
无核酸酶活性（保留DNA结合能力）
转录激活功能完全失效
经工程化改造（RuvC结构域突变）
识别TTTV PAM序列
兼容单crRNA引导
CDS (4860nt):
atgaagcgacctgccgccacaaagaaggctggacaggctaagaagaagaaagctagcagcatctaccaggagttcgtcaacaagtattcactgagtaagacactgcggttcgagctgatcccacagggcaagacactggagaaca
tcaaggcccgaggcctgattctggacgatgagaagcgggcaaaagactataagaaagccaagcagatcattgataaataccaccagttctttatcgaggaaattctgagctccgtgtgcatcagtgaggatctgctgcagaattactcag
acgtgtacttcaagctgaagaagagcgacgatgacaacctgcagaaggacttcaagtccgccaaggacaccatcaagaaacagattagcgagtacatcaaggactccgaaaagtttaaaaatctgttcaaccagaatctgatcgatgct
aagaaaggccaggagtccgacctgatcctgtggctgaaacagtctaaggacaatgggattgaactgttcaaggctaactccgatatcactgatattgacgaggcactggaaatcatcaagagcttcaagggatggaccacatactttaa
aggcttccacgagaaccgcaagaacgtgtactccagcaacgacattcctacctccatcatctaccgaatcgtcgatgacaatctgccaaagttcctggagaacaaggccaaatatgaatctctgaaggacaaagctcccgaggcaatta
attacgaacagatcaagaaagatctggctgaggaactgacattcgatatcgactataagactagcgaggtgaaccagagggtcttttccctggacgaggtgtttgaaatcgccaatttcaacaattacctgaaccagtccggcattactaa
attcaataccatcattggcgggaagtttgtgaacggggagaataccaagcgcaagggaattaacgaatacatcaatctgtatagccagcagatcaacgacaaaactctgaagaaatacaagatgtctgtgctg………….
参考文献
Ming, M. et al. CRISPR-Cas12b enables efficient plant genome engineering. Nat. Plants 6, 202–208 (2020).
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