-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1158 | ShoCas12k mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：ShoCas12k（源自Scytonema hofmannii的V-K型CRISPR-Cas蛋白）
CRISPR类型：Type V-K
RNA引导的DNA转座活性
介导大片段DNA定向整合（T-LR为主）
识别TTTV（V=A/C/G）PAM序列
需与TnsB、TnsC、TniQ组成四组分CAST系统
插入位点位于PAM下游55-75 bp
CDS (1938nt):
atgagcacaatcacgattcaatgccgcctagttgcagaagaagcgaccctgcgctacttttgggaacttatggctgagaagaatacgccactcattaatgaattgttggagcagttaggacaacatccagattttgatacttgggtacaagc
aggcaagatgcctgaaaagacagttgagaacttgtgcaagtccctagaagatcgagaaccttttgcaaatcagcccggtcgttttcgtacttcggcagttgctctggtaaagtacatttacaaatcctggtttgcgttacaaaaacgtcggg
cggacagactagaaggtaaagagcgctggttaaagatgctcaaaagtgatgtggaactggaacgagaaagcaactgtagtttggatattattcgagctaaagctggggaaattcttgctaaagttactgaggggtgcgctccgagcaat
caaacatcctctaagagaaaaaagaagaaaactaaaaaaagccaagcgacaaaagatctccctacgcttttcgagattatattaaaggcttacgaacaagctgaagaaagcctgacacgagctgcccttgcttatctccttaaaaatgact
gtgaagtcagtgaagttgatgaagattcagagaaatttaaaaagcgtcggcgtaaaaaggaaatagaaattgaacgtttaagaaatcaactgaaaagtcggatacctaaaggtcgcgatttaaccggagataagtggttgaaaacgctag
aagaagccacccgaaatgttcctgaaaatgaggatgaggcaaaggcttggcaagcccaactcttgagagaagctagttctgttccttttcctgtagcttatgaaactagtgaagatatgacttggtttaccaacgagcaaggtcgaatcttt
gtttactttaatggttcagccaaacataaatttcaagtttattgcgatcgccgtcaacttcattggtttcaacgttttgtagaggactttcaaatcaagaaaaatgg………….
参考文献
Tou, C. J. et al. Precise cut-and-paste DNA insertion using engineered type V-K CRISPR-associated transposases. Nat. Biotechnol. 41, 968–979 (2023).
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