-80℃储存，低温运输。
包装内容：
产品编号 | 产品名称 | 包装 |
N1172 | PsaCas12f mRNA (1mg/ml) | 100/1000μg |
产品简介：
名称/别名：PsaCas12f（Pseudomonas aeruginosa Cas12f）
CRISPR类型：Type V-F
肽链长度：约700-800氨基酸（分子量~70-80 kDa）
RNA引导的双链DNA靶向切割
依赖T-rich PAM识别（5'-TTN）
超紧凑结构（最小CRISPR效应器之一）
自主crRNA加工能力
产生不对称交错DNA末端
CDS (2502nt):
atgaaacggacagccgacggaagcgagttcgagtcaccaaagaagaagcggaaagtcatgcccagtgagacatacatcaccaagactctcagtctgaagctcatcccttctgacgaggagaagcaggctttggaaaactactttatta
ccttccaacgggccgtaaatttcgccattgatcgaatagtggatatacgttcctctttcagatatctgaataagaatgagcagttcccagcggtctgtgattgttgtggcaagaaggagaaaattatgtacgtgaacatatctaacaaaaccttt
aaattcaagccaagccgaaaccagaaggatcgatacactaaagatatttacaccatcaagccaaacgctcacatttgtaaaacttgttacagcggagtcgccgggaacatgtttatccgcaaacagatgtacccaaacgataaagaagg
atggaaagtttctcgctcctataacataaaagtcaatgctccagggttgactggcaccgaatatgcgatggcaattcggaaggcaatcagtatactgaggtcttttgaaaagcgccgcaggaatgcagaacgcagagcaattgaatacga
gaaatcaaagaaagagtacttggagctcattgacgatgtggagaagggaaagactaacaaaatcgttgtccttgaaaaggaaggccaccaacgggttaaacgttataaacataagaactggcccgaaaagtggcaaggaatttcactg
aacaaagccaaaagcaaagtaaaggatatcgagaaaaggattaagaagctgaaagaatggaaacacccaacactgaatcgtccatatgttgaactccataagaataacgtgcgaatcgtaggctatgagactgttgagctcaaactgg
gaaataagatgtacacaatccatttcgcatcaataagtaacttgagaaagccatttcggaagcaaaagaagaagtccattgaatatctcaagcatttgctcacactcgccctcaagagaaatctggaaac………….
参考文献
Jiang, K. et al. Rapid in silico directed evolution by a protein language model with EVOLVEpro. Science (2024).
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