AdmCas13d mRNA

-80℃储存，低温运输。

包装内容：

产品简介：

名称/别名：AdmCas13d

CRISPR类型：Class 2 type VI-D Cas13d

活性：靶向并切割单链RNA，可用于circRNA敲低

特性：哺乳动物细胞表达强，与RfxCas13d相比活性略低，特异性高，对线性RNA干扰少

应用：circRNA功能筛选、RNA干扰、功能验证

CDS (3729nt):

atgctgtaccctgagcggctgcggcggatcctgaccgctagcaacaataagaggaagaccaaggcaaaggcagcaggcctgaagagcgtgttctttgaccagaagcaggccgtgctgaccacattcgccaagggcaacaatagcc

agatcgagaagaaggtggtgaactccgaggtgaaggatctgagacagccccctgccttcgacctggagctgaaggagaagaccttttacatctctggcaagaacaatatcaacacaagcagggagaatcctctggcctctgccagcc

tgccactgagcaagaggcagcgcatccgggcagagcgcatcaagagagccagggaggagaaccgcccataccacaatgtgaagcgggtgggcgaggacgatctgagagccaaggccgatctggagaagcactacttcggcaa

ggagtattccgacaacctgaagatccagatcatctataacatcctggatatcaataagatcatctctccctacatcaacgacatcgtgtatagcatgaacaatctggccaggaatgacgagtacatcgatggcaagatcgacgtgatcggc

agcctgagctccaccacagattattctagctttatgtccccaaacaaggacctggagaaggagaagaagttctcttttcaccgcgagaattacaagaagttcgtggaggcctccaagccctatatgaggtactatggcaaggtgtttatcc

gcgatgtgaagaagtctaagctgagcaccggcaagggcgagaagatcgaagtgatgtaccggtctgacgaggagatcttcacaatctttcagatcctgagctatgtgagacagtccatcatgcacaacgatatcggcaataagtcctct

atcctggccatcgagaagtaccctgcccggttcgtgggctttctgagcgacctgctgaagaccaagacaaacgatgtgaatagaatgttcatcgacaacaattcccagaccaacttttgggtgctgttctctatctttggcctgcaggatca

cacaagcggcgccgacaagatctgccggaact………….

参考文献

Li, S. et al. Screening for functional circular RNAs using the CRISPR–Cas13 system. Nat. Methods 17, 1207–1216 (2020).

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