FrCas9 mRNA

-80℃储存，低温运输。

包装内容：

产品简介：

来源种属：Faecalibaculum rodentium（罗氏粪杆菌）

蛋白类型：II型CRISPR-Cas系统的RNA引导DNA核酸内切酶

序列特征：与常用的SpCas9序列同源性较低，仅为29.21%，但保留了Cas9家族的核心结构域架构

FrCas9特异性识别5'-NRTA-3' PAM序列，其识别机制与SpCas9存在显著差异

HNH结构域负责切割靶DNA链；RuvC结构域负责切割非靶DNA链

CDS序列（4191nt）

atgggtattcacggggtgcctgcggcttgcaccaaggagtcggaaaagctcaataagaacgcggattactacattggtctggacatgggcacatcctcagctggctgggcggtgagtgactccgagtacaatctaatccgcagaaaag

gcaaggatttgtggggcgttcgccagttcgaagaagcgaaaacggcggcggaacgccgggggttccgggtcgctcgtcgaaggaaacagaggcagcaggtcaggaatcgactattgagtgaggaatttcagaatgaaatcacaaa

gatcgattctgggtttttaaaaagaatggaggattctcggtttgttattagcgacaagagggtgccagagaagtacaccctgttcaatgattccgggtacacagatgtagaatactacaaccagtacccgaccatctaccatctgagaaaag

ccttaatcgaatccaatgagaggtttgatattcgccttgtattcctgggaatccactcgttgttccagcatccaggccatttcctcgacaagggggacgtggatacggacaatacaggtcccgaagaactgatacagtttctcgaagactgc

atgaacgagatacaaatcagcatcccgctcgtctcaaaccaaaaagtcttaactgatatcttgacggatagccgaataacgaggcgtgacaaagaacagcaaattcttgagatattgcaacccaacaaagagtccaaaaaggccgtttc

ccagttcgtgaaggttctcacaggtcaaaaagccaagttaggcgatctcattatgatggaagataaagatacggaggagtacaagtattcattttctttccgcgaaaagaccctggaggagattcttcccgatatagagggcgtcatcgac

ggtcttgcgcttgagtatatcgaatctatctactcgctatattcatggagcctcctcaacagctacatgaaagatacattgaccggtcattattactcgtatctagctgaggcccgcgtggcggcgtacgataagcatcatagtgatcttgttaa

gctgaaaacattgttccgc

参考文献

Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., et al. (2022). FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity. Nat. Commun. 13, 1425. https://doi.org/10.1038/s41467-022-29089-8.

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